Eif4e antibody

Author: mkzu

August undefined, 2024

WebEIF4E Antibodies (Eukaryotic Translation Initiation Factor 4E (EIF4E)) The protein encoded by this gene is a component of the eukaryotic translation initiation factor 4F complex, which recognizes the 7-methylguanosine cap structure at the 5' end of cellular mRNAs. WebAnti-EIF4E Antibody is useful for researcher interested in cancer research and AKT signaling. Application Note. Anti-EIF4E Antibody has been tested in Western Blot and IHC. Expect a band at ~25-28kDa in western blot using appropriate tissues and lysates. Positive control used: HEK293T, MOLT-4 and Daudi lysates in Western Blot, Human Lung ...

Recombinant Anti-eIF4E antibody [EPR19334-137] - BSA and …

WebDec 30, 2016 · eIF4E Antibody (P-2) is an IgG 1 κ mouse monoclonal eIF4E antibody (also designated EIF4E antibody, or EIF4E1 antibody) suitable for the detection of the eIF4E protein of mouse, rat, human and … WebOct 14, 2024 · eIF4E Drives Expression of the Capping Machinery. In order to identify previously unknown eIF4E target transcripts, we leveraged a fundamental difference in the RNA-binding properties of eIF4E in the nucleus and cytoplasm. In the nucleus, eIF4E binds ∼3,000 capped mRNAs which typically are its export targets ( 36 ). how much will fed reduce balance sheet

Phospho-eIF4E (Ser209) Polyclonal Antibody - Thermo Fisher Scientific

WebMouse anti Human EIF4E antibody detects a band of 28 kDa. The antibody has been extensively validated for western blotting using whole cell lysates. Product Details Target Species Human Western Blotting Anti EIF4E detects a band of approximately 28 kDa in Jurkat cell lysate. Species Cross-Reactivity WebMar 25, 2024 · Western Blot: eIF4E Antibody [NB100-58833] - Whole cell lysate (50 ug) from HeLa, HEK293T, Jurkat, and mouse NIH 3T3 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-eIF4E antibody used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds. quantity: 0.1 ml price: 429 … WebWestern blot analysis of extracts from HeLa cells, untransfected or transfected with either nonspecific control siRNA or eIF4E siRNA. eIF4E was detected using eIF4E Antibody #9742, and eIF4B was detected using eIF4B Antibody #3592. The eIF4E Antibody confirms silencing of eIF4E expression, and the eIF4B Antibody is used to control for … how much will financial aid cover

Human/Mouse/Rat eIF4E Antibody MAB3228: R&D Systems

Anti-phospho eIF4E (Ser209) Antibody 07-823 - EMD Millipore

WebMar 6, 2024 · Detector: ab277240, Human eIF4E (pS209) Detector Antibody (unconjugated) The reference range value is 234.375 - 15000 pg/ml. Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for … WebThis product is a recombinant monoclonal antibody, which offers several advantages including: - High batch-to-batch consistency and reproducibility. - Improved sensitivity and specificity. - Long-term security of supply. - … how much will fifa 23 beWebeIF4E in MCF-7 Human Cell Line. eIF4E was detected in immersion fixed MCF-7 human breast cancer cell line using 10 µg/mL Mouse Anti-Human/Mouse/Rat eIF4E Monoclonal … men\u0027s weight chart by age cdc

"WebeIF4E Antibody detects endogenous levels of total eIF4E protein. The antibody does not cross-react with other proteins. Species Reactivity: Human, Mouse, Rat, Monkey, Mink, Zebrafish Source / Purification … " - Eif4e antibody

Eif4e antibody

WebAntibody was affinity purified using an epitope specific to 4E-t/eIF4E-T immobilized on solid support. The epitope recognized by A300-706A maps to a region between residue 925 and the C-terminus (residue 985) of human Eukaryotic Translation Initiation Factor 4E Nuclear Import Factor 1 using the numbering given in entry NP_062817.1 (GeneID 56478). WebeIF4E is involved in several cellular processes including enhanced translational efficiency, splicing, mRNA stability, and RNA nuclear export. All eukaryotic cellular mRNAs are blocked at their 5-prime ends with the 7 …

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WebBackground Information. Eukaryotic translation initiation factor 4E (EIF4E) is the rate-limiting part of mRNA 7-methyl guanosine cap … WebAntigen: Eukaryotic Translation Initiation Factor 4E (EIF4E) Catalog number: ABIN1386772 Lot number: 140106 Secondary Antibody Antibody: Biotinylated goat anti-rabbit/anti …

WebHuman eIF4E ELISA Kit (ab214564) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of eIF4E protein in cell culture extracts and tissue extracts. It uses our proprietary SimpleStep ELISA® technology. Quantitate Human eIF4E with 34.69 pg/ml sensitivity.

WebFor the independent antibodies validation the evaluation is based on comparison of the staining of two (or more) independent antibodies directed towards independent epitopes … WebJul 20, 2024 · eIF4E phosphorylation modulates pain and neuroinflammation in the aged eIF4E phosphorylation modulates pain and neuroinflammation in the aged Geroscience. 2024 Dec;42 (6):1663-1674. doi: 10.1007/s11357-020-00220-1. Epub 2024 Jul 1. Authors Prapti H Mody 1 , Natalia L Dos Santos 1 , Luz R Barron 1 , Theodore J Price …

WebEukaryotic translation initiation factor 4E (EIF4E) is the rate-limiting part of mRNA 7-methyl guanosine cap-binding component of the translation initiation complex eIF4F. The other subunits of EIF4F are a 50-kD …

WebPolyclonal Antibody for studying eIF4E. Cited in 199 publications. Validated for Western Blotting, Immunohistochemistry (Paraffin). Available in 2 sizes. Highly specific and rigorously validated in-house, eIF4E Antibody (CST #9742) is ready to ship. how much will fox nation streaming costWebMore>> Anti-phospho eIF4E (Ser209) Antibody is an antibody against phospho eIF4E (Ser209) for use in WB. Less<< Anti-phospho eIF4E (Ser209) Antibody MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and … men\u0027s weight classes ufcWebEIF4E (EIF4E1, EIF4EL1, EIF4F) protein expression summary. Human assay: BJ [Human fibroblast] fixed with PFA, dilution: 1:139 Human assay: U-251MG fixed with PFA, dilution: 1:139 how much will free college cost taxpayersWebEIF4E is a eukaryotic translation initiation factor involved in directing ribosomes to the cap structure of mRNAs. It is a 24-kD polypeptide that exists as both a free form and as part of a multiprotein complex termed … how much will fortnite nfl skins costWebAntibodies that detect eIF4E can be used in several scientific applications, including Western Blot, Immunohistochemistry, Immunocytochemistry, Flow Cytometry and … men\\u0027s weekender bag with shoe compartmentWebPhospho-eIF4E (Ser209) Antibody (PA5-17919) in ICC/IF. Immunofluorescence analysis of Eukaryotic translation initiation factor 4E was performed using 70% confluent log phase HeLa and HeLa cells, serum starved (SS) for 16 hours and serum released (SR) with 20% FBS for 30 minutes. The cells were fixed with 4% paraformaldehyde for 10 minutes ... how much will foldimate costWebeIF4E is a protein that modulates the translation of maternal mRNAs in early embryos before the onset of zygotic transcription. eIF4E also influences the overall rate of translation. . … how much will food stamps go up in 2022