WebI do live cell imaging and want to stain actin and view it under the microscope in the living cells. My challenge is that my cells of interest are primary cells that only survive for up to 12 hours so transfection with actin constructs will not work. WebPhalloidin is a mushroom-derived fluorescinated toxin which can be used to label F-actin of the cytoskeleton (λ=495 nm, λ=520 nm). ￥1280 /毫克 武汉科斯坦生物科技有限公司 (产品目录 5000+) VIP 4年 备 在线询价 洽谈 1个类似产品 毒蠅虎蕈鹼,90%,17466-45-4 北京沃凯生物科技有限公司是国内业务较为全面、产品与 服务较为专业的优质生命科学公司之一。 …

Are there any stains for actin that will work in living cells ...

Phalloidin belongs to a class of toxins called phallotoxins, which are found in the death cap mushroom (Amanita phalloides). It is a rigid bicyclic heptapeptide that is lethal after a few days when injected into the bloodstream. The major symptom of phalloidin poisoning is acute hunger due to the destruction of liver cells. It functions by binding and stabilizing filamentous actin (F-actin) and effec… WebFITC (Ex-Max 494 nm/Em-Max 520 nm): Fluorescein isothiocyanate - has a very high efficiency of energy transfer from absorbed to emitted light and is one of the most … early 2008 macbook pro keyboard

Phalloidin staining protocol Abcam

WebWash the cells 2–3 times in PBS. 2.4 Add 100 μL/well (96-well plate) of Fluorescein Phalloidin working solution into the fixed cells, and stain the cells at room temperature for … WebPhalloidin, a toxin isolated from the death cap mushroom, Amanita phalloides, binds to filamentous actin with high affinity, and this has made fluorophore-conjugated … WebDE69831888T2 DE69831888T DE69831888T DE69831888T2 DE 69831888 T2 DE69831888 T2 DE 69831888T2 DE 69831888 T DE69831888 T DE 69831888T DE 69831888 T DE69831888 T DE 69831888T DE 69831888 T2 DE69831888 T2 DE 69831888T2 Authority DE Germany Prior art keywords strain coli pathogenic mutant … css table font size