Head kidney macrophages

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August undefined, 2024

WebDec 1, 2024 · In addition, the head kidney macrophages (HKMs) in turbots were isolated and incubated with VD3 in vitro, the results showed that VD3 significantly promoted the … WebBy day 29 head kidney macrophages were isolated and subjected to in vitro poly I:C stimulation to simulate viral infection, and samples were collected over 48 h. By day 58 fish were subjected to in vivo stimulation using poly I:C or a Vibrio water-based vaccine to simulate viral or bacterial infection, respectively. The fish were monitored for ...

M2 macrophages in kidney disease: biology, therapies, and

WebDec 2, 2024 · Macrophages are heterogenous cells of the innate immune system that can fluidly modulate their phenotype to respond to their local microenvironment. They are found throughout the renal compartments, … WebAbstract. Yersinia ruckeri is the etiological agent of enteric redmouth disease, a systemic infection which mainly affects salmonids. Although this important freshwater pathogen was discovered in 1966, little is known about its virulence mechanisms. In the present study, the interactions with rainbow trout head kidney macrophages were investigated. money watch comedy

Head Kidney-Derived Macrophages of Common Carp (Cyprinus …

WebJan 11, 2024 · a The head kidney is dominated by hematopoietic interstitial tissue. Note the abundance of melano-macrophages (black). Endocrine tissue (adrenal homolog) is marked with asterisk. b The transition between head kidney (HK) and trunk kidney (TK) is marked with a red, dotted line. Note the appearance of glomeruli and tubuli in the trunk kidney. WebSep 28, 2015 · Unlike the predominantly intracellular release of ROS and NOS in monocytes and macrophages, teleost neutrophils elicit robust responses both intracellularly and extracellularly [57,60]. ... In addition, the parasitic haemoflagellate, Trypanoplasma borreli has also been shown to induce NO production in head kidney neutrophils of carp [43,57]. WebIn mammals, HSCs originate from the bone marrow, while in fish, the primary hematopoietic organ is the anterior (or head) kidney. A heterogeneous population of adherent leukocytes, containing monocytes and macrophages, amongst other cells, can be isolated from the head kidney using Percoll density gradient centrifugation (21, 25, 26). moneywatch moneyist

Persistence of Yersinia ruckeri in trout macrophages - PubMed

Anatomy of teleost fish immune structures and organs

WebJul 1, 2005 · Previous studies have demonstrated that beta-glucans stimulate Atlantic salmon, Salmo salar L., head kidney macrophages both in vitro and in vivo and increase protection against various pathogens. WebIn the present study, we have thoroughly investigated butyrate-induced production and function of IL-22 in fish macrophages. Our results demonstrated that short-chain fatty acids (SCFAs), major microbiota-derived metabolites, promoted the expression of IL-22 in head kidney macrophages (HKMs) of turbot (Scophthalmus maximus L.). moneywatch lexington kyWebIn conclusion, two distinct macrophage cell lines have been established for the first time from the head kidney of marine fish, which could be useful for studying immunity … money watch canada

"WebFeb 1, 2024 · In the current study, we isolated macrophages from turbot head kidney, which is the largest organ of hematopoiesis in teleost fish until adulthood, comparable to the bone marrow in mammals [20]. Our analysis by RT-PCR and Western blot analysis confirmed that SCFAs induced IL-22 expression in fish macrophages, contributing to … " - Head kidney macrophages

Head kidney macrophages

Kidney-resident macrophages—a role for healing …

WebOct 10, 2024 · The morphology of head kidney macrophages from the Caspian Kutum (a) and the Persian sturgeon (b) after 24 h of culture.Formation of giant cells in the Caspian kutum (c) and the Persian sturgeon (d) after 5 days of culture.E. coli was phagocytized by macrophages from Caspian kutum (e), and Persian sturgeon (f).Aggregations around … WebThe salmonid head kidney is a hematopoietic organ and contains many T and B lymphocytes, macrophages, and melano-macrophages [50,51,52]. Interestingly, the SHK-1 cell line can synthesize melanin [ 53 ], and melanogenesis pathway genes have shown a high expression level in salmon head kidney and SHK-1 cells [ 51 , 53 ].

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WebM2 macrophages are usually termed in the literature as anti-inflammatory and wound healing. Substantial progress has been made in elucidating the biology of M2 … WebApr 14, 2024 · Carbon nanotubes (CNTs) have gained much attention due to their superb properties, which make them promising options for the reinforcing composite materials with desirable mechanical properties. However, little is known about the linkage between lung exposure to nanomaterials and kidney disease. In this study, we compared the effects …

WebAug 30, 2024 · Here, we showed that the head-kidney macrophages in grass carp were capable of engulfing large amounts of Hb. The incubation with Hb increased the level of intracellular reactive oxygen species (ROS) in head-kidney macrophages through the Fenton reaction of Hb. The results of quantitative real-time PCR and RNA-seq analyses … WebAcute kidney injury (AKI) is defined by a rapid decline in renal function. Regardless of the initial cause of injury, the influx of immune cells is a common theme during AKI. ... The …

WebDuring Vibrio harveyi infection, the gut-liver axis was affected, since intestinal inflammation and dysbacteriosis were observed concomitantly with the appearance of hepatic nodules, besides the typical symptoms in the heart and kidney (Deng et al.). Head kidney macrophages of Atlantic salmon fed with a fish meal and fish oil diet, showed the ... WebMar 1, 2016 · poNLRC3 gene is ubiquitously expressed in all tested tissues and is dominantly expressed in the Japanese flounder head kidney macrophages (HKMs). We for the first time showed that poNLRC3 expression was significantly modulated by the stimulation of extracellular ATP, an important danger/damage-associated molecular …

WebApr 13, 2024 · The analysis of ROS production and phagocytic activities in head kidney macrophages upon (r)OnTRAP5b. Head kidney macrophages were stimulated with 100 or 500 μg/mL (r)OnTRAP5b or (r)MBP, and oxidation of DCFH-DA was measured for 30 min using excitation and emission filters of 488 and 525 nm, respectively (A).

WebThis study examined the transcriptional patterns of 11,388 AK leukocytes using 10× Genomics single-cell RNA sequencing and identified five distinct immune cell subsets, which included B cells, T cells, granulocytes, macrophages, and dendritic cells (DCs), which were not identified in more detail according to the known specific markers. money watching moviesWebDec 15, 2024 · To further explore the immune potentiation mechanism of SWCNTs-M-G2, we performed corresponding in vitro experiments. G2, M-G2, SWCNTs-G2 and G2SWCNTs-M-G2 with fluorescent label (FITC) were co-incubated with isolated head kidney macrophages of largemouth bass, respectively, and immunofluorescence … money watch liveWebApr 24, 2014 · The role of calcium (Ca 2+) and its dependent protease calpain in Aeromonas hydrophila-induced head kidney macrophage (HKM) apoptosis has been reported.Here, we report the pro-apoptotic involvement of calmodulin (CaM) and calmodulin kinase II gamma (CaMKIIg) in the process.We observed significant increase in CaM … money watch incWebMelanomacrophage centers (MMCs) are aggregates of highly pigmented phagocytes found primarily in the head kidney and spleen, and occasionally the liver of many vertebrates. Preliminary histological … moneywatch sdn bhdWebThe effects of these recombinant proteins were assessed in vitro using head kidney macrophages of gilthead seabream (Sparus aurata L., Perciformes, Sparidae) and rainbow trout (Oncorhynchus mykiss W., Salmoniformes, Salmonidae). In both species, 3 h of stimulation with rFla and rND1 induced expression of the proinflammatory cytokines ... moneywatch retirementWebThe head kidney functions not only as a primary lymphoid organ, but also, as a secondary lymphoid organ rich with monocytes and macrophages that are involved in phagocytosis of invading pathogens and secretion of cytokines, such as the pro-inflammatory cytokines … Catecholamines (CAs) are essential biogenic amines that are present in … Lipopolysaccharide. Lipopolysaccharide (LPS) is an endotoxin present in the … moneywatch marketWeb1 day ago · The role of microRNAs (miRNAs) in Mycobacterium fortuitum pathogenesis is not well illustrated. Using zebrafish kidney macrophages (ZFKM) we observed that M. fortuitum triggers miR-155 expression, and the TLR-2/NF-κB axis plays a key role in initiating the process. We report that mir-155 activates the pro-inflammatory Stat1/TBX21 … money watch newsletter