Phis2.1 vector

Author: fhyb

August undefined, 2024

WebbpHis2载体价格650元，pHis2载体质粒图谱(Vector map),载体序列(Sequence)见下文，载体质粒抗性为Kanamycin，质粒大小为7207bp。pHis2是酵母单杂交载体。 Webb1.一袋培养基干粉倒入三角瓶或培养瓶中，加0.5L去离子水中溶解（琼脂冷水不溶）。 2.无调整pH值，121℃高压灭菌15min。 3.液体培养基封好口避光、室温储存备用。 4.固体 …

bacterial vectors

Webb1 年: 应用: 酵母双 ... pHIS2 vector酵母单杂交克隆载体 2μg MF2354-1000UL Y187 Chemically Competent Cell 酵母菌化学感受态细胞 10×100μl MF2356-1000UL AH109 Chemically Competent Cell酵母菌化学感受态细胞 10×100μl MF2358-1000UL EGY48 Chemically Competent ... WebbpHIS2 Vector Information PT3705-5 Sold as part of Catalog No. 630304 Restriction Map and Multiple Cloning Site (MCS) of pHIS2 Vector. Unique restriction sites are in bold. … dewalt 18v battery charger instructions

Phis2 1 TaKaRa Bioz

Webb方法通过克隆 MaAQP1 的启动子，将启动子构建到pHIS2诱饵质粒上，转化酵母菌构建诱饵表达载体，同时构建干旱胁迫的香蕉（ Musa acuminat L. AAA group cv. Brazilian）cDNA文库。结果克隆获得1 362 bp的启动子序列，通过分析其顺式作用元件，结果显示启动子序列中共有72个顺式作用元件，包括了TATA-box和CAAT-box核心元件，ABA响应元件 … Webb质粒简介 pHIS2 is a reporter vector that can be used in yeast one-hybrid assays to identify and characterizeDNA-binding proteins. The vector was specifically designed for use with … WebbLower panel revealed that pHis2.1 vector encompassing FL, ∆1, or ∆2 promoter region of the ANAC003 gene was amplified, respectively by using the primer set located at the … dewalt 18v battery chargers

(PDF) Exploring transcriptional signalling mediated by OsWRKY13, …

Webb27 mars 2024 · 筛选含有报道质粒的酵母细胞具体过程为：合成靶序列；将靶序列插入pHIS2载体的多克隆位点；一般为将0.1mg pHIS2质粒用EcoRⅠ和SacⅠ进行完全双酶 … WebbA yeast one-hybrid library was constructed to screen the binding proteins of suspensor specific cis-element. In this study, five repeats of suspensor specific cis-element … dewalt 18v battery dc9096 2Webb24 aug. 2024 · The promoter of each gene was cloned and inserted into the pHis2.1 vector to generate reporter constructs. TcMYC2 was fused to the GAL4 activation domain to … church invitation to worship

"Webb1.构建载体目的基因融合连入pGADT7-Rec2(AD); 顺式作用元件连入pHis2或pHis2.1载体(顺式元件可以只连接一个拷贝，也可以连接3个串联的并测序确认无误)。 2.验证两载体分 … " - Phis2.1 vector

Phis2.1 vector

WebbChina Agricultural University (CAU, Chinese: 中国农业大学; pinyin: Zhōngguó Nóngyè Dàxué; abbreviated 农大/Nóngdà) is a university in Beijing, People's Republic of China specializing in agriculture, biology, engineering, veterinary medicine, economics, management, humanities and social science. WebbThe target DNA sequence, or Bait Sequence, is cloned into pHIS2.1 as one copy or tandem repeats. Interaction between a DNA-binding protein and the target sequence stimulates …

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WebbpHIS2 vector (V001297#) Basic Vector Information. pHIS2 is a reporter vector that can be used in yeast one-hybrid assays to identify and characterize DNA-binding proteins. The … Webb6 feb. 2024 · Lower panel revealed that pHis2.1 vector encompassing FL, Δ1, or Δ2 promoter region of the ANAC003 gene was amplified, respectively by using the primer …

Webb22 juli 2013 · Description. pHIS2 is a reporter vector that can be used in yeast one-hybrid assays to identify and characterize DNA-binding proteins. The vector was specifically … http://www.honorgene.com/product_list/carrier_library/123112.html

WebbStandard cloning vector (phagemid excised from lambda ZAPII). The f1 (+) orientation allows rescue of sense strand ssDNA. pBluescript II KS(+) ... The 1-base overhangs … WebbpHIS2 Description pHIS2 is a reporter vector that can be used in yeast one-hybrid assays to identify and characterizeDNA-binding proteins. The vector was specifically designed for …

WebbpTALEN v2 (NN) pTALEN v2 (HD) pX335 pX260 pX320 pShuttle-CMV pShuttle pAdTrack-CMV pAdTrack pAdEasy-1 BJ5183 AdEasy-1 pAd/BLOCK-iT-DEST RNAi Gateway Vector pDC315 pBHGloxdelE13cre pShuttle-IRES-hrGFP2 pAAV-MCS pAAV-RC2 pAAV-RC pHelper pAAV-LacZ pAAV-IRES-hrGFP pCMV-MCS RNAi-Ready pSIREN-RetroQ RNAi-Ready …

http://www.honorgene.com/uploads/files/202411/6181dfe1813d2.pdf church invitehttp://www.zoonbio.com/molecular/yeast-one-hybrid-procedure.html dewalt 18v battery charger home depotWebbIn this construct, three copies of the DNA target (T) have been inserted into the pHIS2.1 reporter vector. GAL UAS minimal promoterADE2, HIS3, lacZ, and MEL1 transcription … church invoiceWebbpHIS2 is a reporter vector that can be used in yeast one-hybrid assays to identify and characterize DNA-binding proteins. The vector was specifically designed for use with the … dewalt 18v battery dc9181Webb13 juni 2015 · GTP1-GTP8 indicates the pGAD-OsGRAS23 plus pHIS2.1-cis (promoters of Os07g0162450, Os01g0537250, Os07g0638400 ... the promoter sequences were cloned into the yeast expression vector pHIS2.1 between the SmaI and EcoRI sites. The constructs were co-transformed into the yeast Y187 (MATa; ura3–52; his3–200; … dewalt 18v battery cell replacementWebb20 feb. 2024 · The full-length cDNA of TcbZIP60 was cloned into the pGADT7 vector used for homologous recombination, and the promoter sequences of TcCHS and TcAOC were cloned separately into the pHis2.1 vector. The resultant pGADT7: TcbZIP60 plasmid was co-transformed into yeast strain Y187, together with pHis2.1: TcCHS or pHis2.1: TcAOC , … dewalt 18v battery dw9099WebbDescription pHIS2 is a reporter vector that can be used in yeast one-hybrid assays to identify and characterize DNA-binding proteins. The vector was specifically designed for use with the BD Matchmaker™ OneHybrid Library Construction & Screening Kit (#K1617-1). church invite cards ideas