WebSep 17, 2024 · The Cre/Esr1 is constitutively expressed, but only translocates to the nucleus when bound to TAM’s metabolite 4-OHT where it then recombines LoxP sites. In the case of this YFP reporter, the... Web162387. pGP-20XUAS-IVS-Syn21-jGCaMP8m-p10. Gal4 activated transgene expression of ultrafast protein calcium sensor under the hsp70 promoter in D. melanogaster. Loading... 162386. pGP-20XUAS-IVS-Syn21-jGCaMP8s-p10. Gal4 activated transgene expression of ultrafast protein calcium sensor under the hsp70 promoter in D. melanogaster.
The mouse elongation factor-2 gene: Isolation and …
WebMar 9, 2024 · The Cre/loxP system is a powerful tool for generating conditional gene knockout (KO) mice and elucidate gene function in vivo. CD19-Cre and Mb1-iCre transgenic mice are commonly used for... WebCre-loxp system is powerful tool for tissue-specific Knockout (KO) and overexpression in vivo and in vitro. Cre-loxP system is widely used in the field of biosciences, especially in the generation of genetically engineered mice (knockout or overexpression), enabling researchers to study the function of gene of interest (GOI). small scale food producers association
Utility of progesterone receptor‐ires‐Cre to generate conditional
WebIn eukaryotes, promoters usually have the following four components: the TATA box, a TFIIB recognition site, an initiator, and the downstream core promoter element. ... Mutations arising within a CRE can generate expression variance by changing the way TFs bind. Tighter or looser binding of regulatory proteins will lead to up- or down-regulated ... WebApr 11, 2024 · For α5-i-KO mice, Cre recombinase was driven by the interneuron-specific GAD2 promoter to target the floxed Gabra5 gene. For α5-pyr-KO mice, Cre was driven by the pyramidal neuron-specific CaMKIIα promoter. B) Western blot analysis of α5-GABA A R (55 kDa) expression in male and female WT, p-WT, α5-i-KO, and α5-gl-KO mice. Expression ... WebTransgenic mouse strains using promoter fragments of the human surfactant protein C (SP-C) and rat club cell secretory protein (CCSP) genes to express Cre in alveolar type II and club cells, respectively, were generated more than a decade ago (1, 2). In these strains, which are widely used to dissect spatiotemporal gene functions related to ... highpoint service network lowyat